Harvested infected cells are treated to remove cellular components and ultracentrifuged to remove whole virus particles. Pure hexon antigen is obtained from the resulting supernatant by ion exchange chromatography and the antigen presented in 30mM BIS Tris Propane buffer, at pH 6.8 containing sodium chloride.
Whole infective virus particles removed by ultracentrifugation and chromatography. Confirmation by attempted growth under original culture conditions.
mg/ml by absorption at 280nm
Frozen (-70ºC). Sonicate before use. Avoid repeated freezing and thawing