Pertussis Toxin is highly purified from Bordetella pertussis culture supernatant and presented in Tris/HCl buffer, pH 8.0, 0.5M NaCl and 50% glycerol.
Determined by UV absorption at 280nm
Whole Bordetella pertussis organisms are removed by sterile filtration. Absence of viable B. Pertussis is confirmed negative by absence of growth under original culture conditions. Since no procedure can guarantee absolute sterility the reagent should be handled with appropriate safety precautions.
This preparation for in-vitro use only. Not for administration to humans.