The Borrelia afzelii organism (Strain PKo) is cultured in broth suspension. Washed harvested organisms are solubilised with detergent to produce a lipoprotein and cytoplasmic cylinder extract enriched with Outer Surface Proteins (OSP).
Presented in PBS buffer containing 1% N-octylglucoside.
1-5 mg/ml by Bradford (Biorad) assay using BSA as standard.
By detergent treatment. Confirmation by attempted growth under the original culture conditions.
-70°C (or lower). Avoid repeated freezing and thawing.
Suitable for ELISA, lateral flow and other solid phase formats. It is recommended that this antigen preparation is sonicated before use.