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Product Catalogue

Neuron-Specific Enolase (NSE), Highly Purified BF 3063

Product Details
Data Sheet
PRODUCT CODE
BF 3063
SHELF-LIFE 1 year from date of manufacture
SOURCE Human brain
PRODUCT PRESENTATION Presented liquid in 0.1M Tris-HCl buffer containing 5 mM Mg2SO4 and 0.25M KCl, pH 5.1
SUBUNIT COMPOSITION Gamma-gamma
PURITY > 95% (SDS-PAGE)
Purified by ion-exchange chromatography and gel filtration.
Immunological identity confirmed by positive reaction in an ELISA with anti-NSE antibodies
PROTEIN CONCENTRATION As determined by spectrophotometry at 280nm using an extinction coefficient of 0.895
SPECIFIC ENZYME ACTIVITY

Measured in U/mg protein

One enzyme unit (U) is defined as the amount of enzyme that converts one micromole of 2-phosphoglyceric acid to phosphoenolpyruvate per minute at 25ºC (ref: J. Biochem, 87, 1587-1594, 1980). Enzyme activity is determined in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG

HBsAg STATUS A blood sample from the tissue donor was tested negative
ANTI-HIV-1/2 STATUS A blood sample from the tissue donor was tested negative
ANTI-HEPATITIS C STATUS A blood sample from the tissue donor was tested negative
STORAGE -70ºC
Avoid repeated freeze-thaw cycles
Neuron-Specific Enolase (NSE), Highly Purified BF 3063
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